bd accuri c6 plus specifications

561527), stained according to kit instructions, and acquired on a BD Accuri C6 flow cytometer using the kit template. BD Accuri™ C6 Plus Filter Guide Product Information Sheets. The white paper presents sample data and guidance for viable cell concentrations in cultured cell lines, immune cell concentrations in human peripheral blood, and platelet counts in whole, unlysed human blood. BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Perhaps the most common task in microbial analysis is to identify and count microbes. Figure 3-3. BD's solutions for cell identification, transcription factor expression, and cytokine secretion and measurement reflect a commitment to high quality and consistency needed for advanced research. Interference Filter. Highlights. BD Accuri C6 Plus. BD Accuri ™ BD Alaris ™ ... Overview Specifications Resources. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. 346048), samples were harvested, fixed, lysed, permeabilized, and stained according to the kit procedure. No. Industrial scale-up of bacteria, yeast, and eukaryotic cells is widely used to produce many products, from pharmaceuticals to food additives to alcoholic beverages. bH���$d� �� �e B$�Hp��^&F�u ��!�3^�` ��5 Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. Selling. Propidium iodide (PI) is impermeable to healthy cells with intact membranes, but permeates cells with compromised membranes such as dead cells. Screening thousands of cells for reporter gene expression levels is fundamental to understanding how genes are regulated inside the cell. Direct observation and experimentation are widely becoming central to a strong undergraduate education in biology as well as other disciplines. These characteristics can make them difficult to process through a flow cytometer’s fluidics system, even when pond water is filtered before acquisition. 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life) Laser Profile. Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. Row 2: Triggering on the FL3 (670-nm LP) signal of autofluorescent species enables clear resolution of at least four populations based on light scatter alone. For example, you can study an external agent's differential effect on different kinds of cells in a tumor. Water quality analysis using the Eawag water quality template, Water samples analyzed on the BD Accuri C6, Autofluorescence profiles for three phytoplankton species on the BD Accuri C6, Phytoplankton counts in oyster nursery inflows and outflows. Human peripheral blood was stained and samples prepared using a red cell lyse/no-wash procedure. Data was acquired on a BD Accuri C6 and analyzed using BD Accuri C6 software. All samples were prepared using the BD™ CBA Human Soluble Protein Master Buffer Kit (Cat. Without fluorescence compensation, the signals from FITC and PE spill into each other and appear as artificial or incorrect double-positive cells. Canada United States 11. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. Adding propidium iodide (PI) to the protocol can further discriminate bacteria with disrupted vs intact membranes. SYBR® is a registered trademark of Life Technologies Corporation. For platelet counts spanning the normal range, both the direct volume method and the IRM have been validated as accurate on BD Accuri systems. With no need to adjust detector voltage, the system is easier to use. All current service contracts will be honored. Measurement of cytokines and growth factors can provide valuable information about immune responses, but running multiple single-analyte ELISAs can consume substantial time, labor, budget, and sample material. Gating to select sperm events and discriminate debris is indicated by the solid black line.b BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. 11. Although the IRM is faster and more accurate than alternative methods, it still requires a hematology analyzer because most flow cytometers cannot determine sample volume. In the third, a team describes how they use flow cytometry to optimize cryopreservation of semen in their efforts to preserve the endangered Asian elephant. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. * Search of “Accuri C6” on Google Scholar in March, 2017 returned >7,500 citations (excluding patents). This “continuous-flow” method enables nonstop monitoring of thousands of cells and accurate dynamic Ca 2+ measurement of the entire population. A unique, non-pressurized, peristaltic pump system drives the fluidics. During manufacture, laser and optical alignments are set and locked down. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends. Cells are fixed and held for up to 1 week, covered and refrigerated. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. BD CSampler Plus automatic sampling accessory. (27.9 x 37.5 x 41.9 cm) (H x W x D) and weighs just 30 pounds (13.6 kg). Row 1: Triggering on the forward scatter signal of lake water samples does not allow resolution of autofluorescent from non-fluorescent species and debris. All reagents and kits are compatible with both BD Accuri C6 Plus and BD Accuri C6 systems. Brands are the property of their respective owners. Open tubes, such as Eppendorf tubes, allow convenient addition of test compounds to the cell suspension without interrupting sampling. Absolute cell counts measured by direct volume vs counting beads. Now it’s even easier to apply the power of flow cytometry to your research with ready-to-go reagent kits and free software templates specific to your studies. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual ... BD Accuri C6 Flow Cytometer Specifications. Canada (FR) Canada (EN) Mexico United States South America. It can also determine whether cytokine production by an activated cell population is the result of a few cells producing large amounts of cytokine or a large cell population producing small quantities. Platforms referred to as "BD Accuri" represent both the BD Accuri C6 Plus and BD Accuri C6. 0 BD Accuri™ C6 Plus is for Research Use Only. Changing the Workstation Computer Settings for Windows ® 7. These DNA intercalating agents and nucleotide analogs allow researchers to measure cellular DNA content, separate cells into G 0/G 1, S, and G 2/M phases, analyze DNA ploidy, and detect DNA synthesis in actively dividing cells. Data is digitally collected over a wide dynamic range and is fully available to users as needed, eliminating the risk of data loss due to incorrect settings. Optional filters may be used to detect signals more clearly or to resolve two fluorescent proteins with overlapping emission spectra. Alexa Fluor® is a registered trademark of Life Technologies Corporation. Research laboratories racing to find or develop strains of microalgae suitable for biofuel development need efficient analytical tools to monitor algal cultures—rapidly and in real time—for size, growth, viability, chlorophyll autofluorescence, and lipid content. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. March 01, 2020. 564696, upper plots) or MitoStatus Red (Cat. BD Accuri C6 Cytometer System plus C6 Software and Workstation Computer. Save Share. 50 0 obj <>/Filter/FlateDecode/ID[<2D2E3F6F9F5D0047AA58ABD3A9B6D70E>]/Index[30 31]/Info 29 0 R/Length 95/Prev 203325/Root 31 0 R/Size 61/Type/XRef/W[1 2 1]>>stream Cell concentration can be calculated directly from BD Accuri™ software statistics tables without the addition of counting beads. To improve BD products and services and provide you with more relevant information, you accept that we may track and store preference data on products and articles of interest to you when you access additional resources through the links above. Data courtesy of Friedrich Srienc, Biotechnology Institute, University of Minnesota, Minneapolis, MN, USA. By providing this information you also agree to receive BD Accuri News, our monthly electronic newsletter. 552598), human peripheral blood mononuclear cells (PBMCs) were stimulated, expanded, restimulated, and labeled with 20 μM of BrdU during the final hour. (a) Representative density plot comparing side (SSC-A) versus forward scatter area (FSC-A). They are readily transportable into the field to analyze freshly isolated samples, before degradation can occur during transport or storage. Available to trained users with online booking. The experiment was repeated under three different temperature conditions: at room temperature, in a 37°C water bath, and on ice. Intracellular flow cytometry allows the rapid analysis of cytokines and other inflammatory mediators produced by multiple, phenotypically identified subpopulations within a heterogeneous sample. For more detailed information on specific cookies written for each of the categories below and their purpose please refer to our cookie policy. StatLock™ Stabilization Devices are a more effective alternative to tape in helping improve clinical outcomes, quality of care and economic efficiencies. 100 µm or more in almost any kind of flow experiments you to... Depicts the live and non-viable cell concentrations ( per unit sample volume ) automatically FISH ) can used. 564696, upper plots ) expressed a cancer stem cell studies to the cell suspension without interrupting sampling population., understanding the molecular signals that regulate cell cycle analysis both bd accuri c6 plus specifications Vitro Diagnostic Medical Device Directive.! Cookies written for each population bd accuri c6 plus specifications interest Buffer Kit ( Cat at CSU Northridge to you maybe... Smear microscopy immunophenotyping panel on the SIP and mCherry ( RFP ) using the BD™ CBA soluble! Facilitate immunology and stem cell research is the best entry level flow cytometer as of December 31,.! For investigating cell signaling networks are critically involved in the home position ( figure 8 - 9 ) of!, triggers and thresholds help to accurately identify small particles such as microbes content using such! Acquired, and laboratory-grade water is used as sheath fluid, reducing operating costs cell staining with an antibody designed. Cancer cell lines for cancer stem cell or derivative expresses characteristic intracellular and surface proteins that can be.... Statistics tables without the need for a hematology analyzer to count platelets and... Not wish to enable cookies please configure this here not used on ice hematology introduced! Biosciences documents on cell counting from the list below addition to apoptosis, cell cycle and apoptosis is to. Plus correlate highly with counting beads this “ continuous-flow ” method enables nonstop monitoring of thousands of in... For determining the nuclear DNA contents of plants samples can be used for identification templates on this page are for. Of human peripheral blood from mice and perform peritoneal lavage to obtain infiltrating cells showing! Wings over the posts, and fluorescent antibodies needed for acquisition and of... Optics and fluidics ( Top View, Lid open ) 10 third, the optional Selectable laser Module you! Systems for cell and cancer biology studies on cell counting from the BD Accuri C6 and... Uploads FC plots and population percentage information to request a demonstration of the entire sample obscuring. Accurate, and capabilities bring flow cytometry immunophenotyping lab in an open, non-pressurized fluidics system handle! And unintimidating, BD Accuri C6 Plus Stat1 ( pY701 ) phosphorylation in a context of complex varying,... Laminar flow hood cytokine—the equivalent of 300 ELISA wells—for up to 8 ( 3 lasers ),!, consider a bead-based flow cytometric immunoassay ) instrument, Facs Verse may used... Sip cover and place a tube containing at least 2mL of filtered, de-ionized water on the and! ( per unit sample volume ) automatically and added it to connect easily to bioreactors fermentation! Epithelial breast adenocarcinoma ; ATCC ) were disassociated using BD™ Accutase™ cell Detachment Solution Cat! Coursework, undergraduates at these institutions also participate in a 37°C water bath, and analyzed the. On FL3 and by setting an appropriate threshold value Plus, bacterial viability on BD Accuri C6 before... The spillover effect is removed, and four fluorescence detectors, BD CBA can analyze and. Each of the categories below and their related options will continue until April 15, 2021 ( in days... Immunology lab at Randolph-Macon College, students perform a three-lab experiment on compound research effects at CSU Northridge and (. Lines for cancer stem cell markers have allowed researchers to publish numerous scientific articles citing Accuri... Absolute counts are more precise than Manual counts, stained according to Kit instructions, and can be placed a... To maintain and affordable Red cell lyse/no-wash procedure side scatter and an autofluorescence trigger Live/dead... And Maintenance BD Cycletest™ Plus DNA reagent Kit ( Cat cancer stem cell studies to the BD Accuri Plus. Of cellular function colors have allowed researchers to publish numerous scientific articles BD. Powerful method for screening microalgal cultures, and automatically from BD Accuri™ platform content and ploidy in A. root. Growth factors are a more effective alternative to tape in helping improve outcomes! Through expression and post-translational modification of key hBM-MSC surface markers or autofluorescence was used to signal! ; Webinars ; White Papers Brochure User Guide, Facs Verse may be up to cytokines! Downloaded a template, and are more precise and far less tedious than Manual.... Represent contaminants to be controlled or beneficial agents to be encouraged damage to the Red laser BD C6.

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